In Silico-Based Vaccine Design Against Hepatopancreatic Microsporidiosis in Shrimp

Enterocytozoon hepatopenaei (EHP) is a spore-forming intracellular parasite that produces an economically devastating disease in farmed shrimp called hepatopancreatic microsporidiosis (HPM), known to slow the growth of hosts aquaculture. Spore wall proteins are implicated recognition host cells microsporidia. Many parasites have been demonstrated detect heparin, glycosaminoglycan (GAG) molecule present on cell surfaces, as host. The sequencing and characterization several EHP strains started reveal information regarding pathogen biology, pathogenicity, spore must bind surface infect it, failure do so results ineffective infection. Despite these complications, there still no cure or vaccine for parasites. In this regard, immunoinformatics method was used generate epitope-based against pathogen. immunodominant T-cell B-cell epitopes were identified using EHP. final constructed sequence developed be immunogenic, non-allergenic well better solubility. Molecular dynamics simulation revealed significant binding stability structural compactness. Finally, Escherichia coli K12 model, codon optimization yielded ideal GC content higher CAI value, which then included cloning vector pET2+ (a). Altogether, our outcomes imply proposed peptide may good option HPM prophylaxis. HIGHLIGHTS Hepatopancreatic (HPM) There approved drugs vaccines control In-silico approach has some advantage previously fishes design candidate would advantages combat sustainable production GRAPHICAL ABSTRACT